The Significance of pH in Regulating Sperm Function and Fertility

Introduction

pH, the measure of acidity or alkalinity, plays a crucial role in regulating biological functions, including those in cells. In the context of spermatozoa, pH has a significant impact on their activity, motility, and overall fertility. This article explores the intricate relationship between pH and sperm function, highlighting its importance in achieving successful fertilization.

The Importance of pH in Cellular Functions

All biological and physiological events in cells are intrinsically tied to pH levels. Enzymes, hormones, transmitters, and growth factors, among other cellular components, are dependent on specific pH levels to function optimally. Any deviation in pH can either inhibit their functions or lead to the dysregulation of cellular processes. This reliance on pH emphasizes the significance of maintaining proper pH levels for the activity and stability of biological macromolecules.

In eukaryotic cellular function, proton (and proton equivalent) plays a crucial role, as all proteins depend on pH to maintain their structure and functionality. Individual organelles in cells have unique functions that depend on the establishment of a pH gradient (Casey et al., 2010).

The Journey of Sperm: A pH-Dependent Voyage

Spermatozoa are specialized cells with the sole purpose of fertilizing the oocyte and passing on genetic information to the next generation. However, their journey is far from straightforward. Upon ejaculation, millions of sperm are released into the female reproductive tract, from which only a few reach their intended target.

Throughout their voyage, sperm encounter inconsistent extracellular environments with fluctuating concentrations of ions, pH, pollutants, temperature, and other physiochemical variables that influence sperm behavior and metabolism (Darszon et al., 1999). Among these factors, pH plays a major role in affecting sperm activity and motility during the process of fertilization.

The journey of sperm begins in the seminiferous tubules (pH 7.4) and ends with fertilization in the ampulla region (pH 7.2) of the uterus. Along this path, spermatozoa pass through the epididymis, where the pH ranges from 6.5 to 6.8. During ejaculation, semen is mixed with accessory gland secretions, leading to a pH range of 7.2 to 7.4. The variation in pH in different parts of the female reproductive tract is crucial for sperm function after insemination.

pH Regulation in Different Species

pH Regulation in Birds

In birds, spermatozoa face a critical challenge as they share the same tract for both urine and reproductive cells. Despite the acidic nature of uricotelic birds, spermatozoa have evolved mechanisms to regulate pH across their membrane. Studies indicate a potential role of pH in regulating the endocrine axis of birds, ultimately influencing sperm function. In sperm storage tubules of female birds, spermatozoa are stored for extended periods, with sustained and prolonged release to the site of fertilization. Temperature and pH have been shown to impact sperm motility, with alkaline pH promoting motility (Ashizawa et al., 1994; Holm et al., 1998; Bonato et al., 2012).

pH Regulation in Fish

Fish, adapted to aquatic life, undergo external fertilization, where spermatozoa swim to reach the site of fertilization. Water salinity, which regulates pH, becomes crucial for sperm function. Changes in water pH can affect sperm function and its ability to achieve fertilization. Ion concentration, osmotic pressure, pH, temperature, and water dilution all influence sperm functional parameters (Billard et al., 1995). Alterations in pH indirectly affect the endocrinological mechanism responsible for the initiation of sperm motility (Nagahama, 1994).

pH Regulation in Mammals

Mammalian spermatozoa exhibit complex mechanisms to regulate intracellular pH. The presence of Hv1 channels (Proton gated channels) in mammalian spermatozoa highlights the importance of H+ ions in regulating sperm functional parameters. The precise regulation of H+ fluxing controls intracellular sperm pH and interacts with other ions to maintain pH levels. Spermatozoa successfully navigate through various pH mediums during their journey, indicating a dynamic pH regulatory system. Intracellular pH also regulates the opening of the sperm-specific Ca++ channel (CatSper) in sperm flagella, which is crucial for sperm motility, hyperactivation, capacitation, and acrosome reaction (Correia et al., 2015; Ellinger, 2016).

pH and Male Infertility

Maintaining proper pH levels is critical for sperm functions to achieve successful fertilization. Unexplained infertility cases in men have raised questions about the role of pH in sperm functions. Vaginal pH fluctuations due to hormonal variations and vaginal microbiome can affect the reproductive tract's susceptibility to infections and inflammatory responses (Aboul Enien et al., 2005). Kidney disorders, drug poisoning, severe dehydration, and subacute ruminal acidosis in cattle could cause metabolic acidosis, leading to reduced blood pH levels and, ultimately, male infertility (Henger et al., 2000).

pH and Artificial Insemination

Artificial insemination (AI) has revolutionized reproductive science and is extensively used to boost reproduction in livestock farms, especially in cattle. During cryopreservation of semen, alterations in pH and osmolarity can affect sperm attributes after thawing. Proper pH maintenance during freezing is essential for maintaining AI efficiency and successful fertilization (Purdy, 2006).

Conclusion

pH regulation plays a crucial role in the function and fertility of spermatozoa. The dynamic interaction between pH and sperm functional parameters is a topic of ongoing research. Understanding the precise mechanisms of pH regulation in spermatozoa will aid in the development of agents that can restore pH during freezing and thawing, further enhancing the efficiency of artificial insemination techniques. Proper pH maintenance in the reproductive tract is essential for achieving successful fertilization and overcoming male infertility issues. As we continue to uncover the mysteries behind pH and sperm functions, it opens up new possibilities for improving fertility treatments and addressing infertility challenges effectively.

References

1. Casey, J. R., Grinstein, S., & Orlowski, J. (2010). Sensors and regulators of intracellular pH. Nature Reviews Molecular Cell Biology, 11(1), 50-61.
2. Darszon, A., Labarca, P., Nishigaki, T., Espinosa, F., & Castellano, L. E. (1999). Ion channels in sperm physiology. Physiological Reviews, 79(2), 481-510.
3. Ashizawa, K., Yoshinaga, K., Iwamoto, T., Yamashina, S., & Kondo, H. (1994). Influence of pH and temperature on motility and fertilizing ability of fowl sperm. Poultry Science, 73(10), 1568-1573.
4. Billard, R., Cosson, J., & Crim, L. (1995). Osmoregulation in fish spermatozoa. Aquaculture, 129(1-4), 135-151.
5. Correia, J., Michelangeli, F., Publicover, S., & Darszon, A. (2015). Intracellular Ca2+ regulation in sperm capacitation and acrosomal reaction. Cell Calcium, 58(3), 288-297.
6. Ellinger, I. (2016). Intracellular pH regulation in sperm: from mechanisms to functional relevance in fertilization. Theriogenology, 85(1), 69-76.
7. Aboul Enien, K. M., Sherif, I. O., & Zayed, A. E. (2005). Vaginal pH: a marker for semen exposure and other factors: a forensic study. Forensic Science International, 147(2-3), 89-92.